Antibody Recipe: A Step-by-Step Guide to Make Your Own Antibodies at Home

Ingredients

  • 1 cup of primary antibody
  • 1/2 cup of secondary antibody
  • 2 tablespoons of blocking buffer
  • 3 teaspoons of wash buffer
  • 1 teaspoon of detection reagent
  • 1/4 teaspoon of substrate solution
  • 1 dish for incubation
  • 1 set of pipettes for accurate measurements
  • 1 microplate reader for quantification

Steps and instructions

  1. Dilute primary antibody in blocking buffer
  2. Incubate sample with primary antibody
  3. Wash sample with wash buffer
  4. Apply secondary antibody to sample
  5. Wash sample again
  6. Add detection reagent to sample
  7. Wash sample one more time
  8. Add substrate solution to develop signal
  9. Read and quantify results using microplate reader

Tools for making

Recipe overview

This antibody recipe provides a step-by-step guide on how to perform an antibody-based assay. By following the instructions, you will learn how to prepare and apply primary and secondary antibodies, detect signals, and quantify results using a microplate reader. This recipe is essential for researchers and lab professionals looking to accurately analyze specific proteins or molecules in biological samples.

Common questions

  1. What type of antibodies should I use for this recipe? Use a primary antibody specific to your target protein and a secondary antibody that recognizes the primary antibody.
  2. Can I use different concentrations of antibodies? Yes, you can adjust antibody concentrations based on your specific experimental needs, but be sure to optimize for best results.
  3. How should I store the antibodies? Store antibodies according to the manufacturer's instructions, typically at recommended temperatures and avoiding multiple freeze-thaw cycles.

Serving dishes and utensils

  • Pipettes - For accurate measurement of antibodies and solutions
  • Microplate reader - To quantify and analyze antibody binding
  • Incubation dish - To hold samples during antibody incubation
  • Lab coat and gloves - To ensure safety and cleanliness during the experiment
  • Wash bottle - For efficient washing steps in the protocol
  • Timer - To track incubation and washing times accurately

Origin stories

Antibodies, essential components in many scientific experiments and medical diagnostics, were first discovered in the late 19th century by German immunologist Emil von Behring. Behring's work on antibodies earned him the first Nobel Prize in Physiology or Medicine in 1901.

Disclaimer: This recipe was not created by humans and we cannot ensure that it will turn out as expected. We do not guarantee or take any liability for the accuracy of this recipe (including steps, ingredients, nutritional information, and all sections on this page). You should check to make sure you are not allergic to any ingredients and take safety precautions while making this. The images on this page are generated by AI and may not accurately represent the result of making this recipe.